The amount of pigment accumulation led to varying degrees of watermelon flesh color exhibited in one color system, such as pink to red and pale yellow to canary yellow. To be honest, one of the best things… Plant 83 (4), 702–708. Lane 1, 7, 13, 19 and 25 are the D2000 plus DNA marker. Flesh color inheritance and gene interactions among canary yellow, pale yellow, and red watermelon. Restriction enzyme digestion of W04EII08-38, WII04EKpnI-1, and WII04E07-40 (MluI, KpnI, and MboII) was more cost-effective than that of WII04EBsaHI-6 and Phe226 (BsaHI). The genes in the carotenoid biosynthesis pathway perform through different action modes to regulate carotenoid accumulation and flesh color formation. grafting for the cultivation of watermelon under adverse environments. (2015). (2011). (2010). Based on these results, we speculated that the main effective genes could determine the formation of the red color system (pink gradually changing to red) and the yellow color system (from pale yellow to canary yellow). Two kinds of assisted selection markers for red flesh color in watermelon were generated by Bang et al. doi: 10.1007/s10068-012-0068-3, Liu, H., Jiao, J. Q., Liang, X. J., Liu, J., Meng, H. W., Chen, S. X., et al. (2014). Developmental changes in gene expression drive accumulation of β-lycopene and carotene in watermelon. Theor. Only a few foods contain high amounts of lycopene. doi: 10.1179/174329211X13020951739938, Liu, C. H., Zhang, H. Y., Dai, Z. Y., Liu, X., Liu, Y., Deng, X. X., et al. Tomato species with higher levels of lycopene accumulation were used in the process of constructing the phylogenetic tree, and the LCYB genes of watermelon were further related to that of tomato. The lycopene content of 50 commercial cultivars of seeded and seedless red-fleshed watermelons was determined. Z., Davis, A. R., Baloch, A. M., Luan, F. S. (2015). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. The phylogenetic tree was constructed by combining the lycopene genes of melon, watermelon, gourd, and pumpkin in Cucurbitaceae crops with one lycopene gene in tomato. doi: 10.1139/g03-060, Sun, L., Yuan, B., Zhang, M., Wang, L., Cui, M. M., Wang, Q., et al. In our research, different shades of flesh color in one color system validate the same MAS results. doi: 10.1093/jhered/esj023, Han, F. Q., Yang, C., Fang, Z. Y., Yang, L. M., Zhuang, M., Lv, H. H., et al. J. Hered. In all the accessions, COS, LSW-177, 97103 (red flesh), and PI 296341 (white flesh), the expression of Cla005011 did not explain the lycopene accumulation difference between different watermelon accessions. B., O’Halloran, D. M., Cosman, K. M., et al. Cla005011: (A) BioProject: PRJNA221197 (RPKM of 97103), sample DAP10, DAP18, DAP28, DAP34, DAP: days after pollination. Genet. Enjoy the benefits of lycopene by eating more tomatoes and processed tomato products. LCYB clusters I and II contained 13 and 9 genes, respectively. doi: 10.1007/s00122-014-2349-0, PubMed Abstract | CrossRef Full Text | Google Scholar, Capel, C., del Carmen, A. F., Alba, J. M., Lima-Silva, V., Hernandez-Gras, F., Salinas, M., et al. According to our results, the CAPS marker WII04EBsaHI-6 and Phe226 shared the same location, and we also implemented a forward genetics strategy to demonstrate the significance of LCYB in lycopene accumulation in watermelon (red to nonred). 82 (2), 267–279. 141 (5), 434–443. 213 (3), 1208–1221. (2006). MEGA X: Molecular evolutionary genetics analysis across computing platforms. Measurement of lycopene contents in watermelon. 1. Volatile chemical and carotenoid profiles in watermelons [Citrullus vulgaris (Thunb.) The genome data of 4 parental materials and 20 other published watermelon accessions (Guo et al., 2012) were used to extract the sequence of the LCYB gene for sequence alignment and variation detection. Watermelon contained the highest content of lycopene (144.27±0.001 mg/kg) and tomato was the second and contained lycopene (104.699±0.000 mg/kg). Nucleic Acids Res. Genet. Molecular mapping and candidate gene analysis for yellow fruit flesh in cucumber. Considering the flesh color–specific markers and the different polymorphisms of white-fleshed plants, WII04EBsaHI-6, W04EII08-38, WII04EKpnI-1, and WII04E07-40 were selected to construct a MAS approach for red, yellowish, and white flesh color in 81 watermelon accessions. doi: 10.1111/tpj.13838, Gao, L., Zhao, W. H., Qu, H. O., Wang, Q. S., Zhao, L. X. For each color group, five representative watermelon accessions MAS results are displayed in Figure S5. doi: 10.1093/bioinformatics/btp348, Cazzonelli, C. I., Pogson, B. J. Figure 8 Phylogenetic tree analysis of lycopene homologous genes in different Cucurbitaceae crops and tomato. It was also likely that the difference in protein levels or functionality may regulate the color of the fruit (Wang et al., 2016). Appl. Basic local alignment search tool. To distinguish the three types of flesh color (red, yellowish, and white), the four markers were combined for further testing. For tomato ketchup, the range was 116.59 and 254.98 mg/kg FW; for tomato juice, the range was 9.90 and 54.21 mg/kg FW. Biol. *Correspondence: Shi Liu, shiliu@neau.edu.cn; Feishi Luan, luanfeishi@neau.edu.cn, Front. Breed. Plant Sci., 08 October 2019 Genet. Studies on the lycopene content of watermelon germplasm collections are very scarce (Pinto et al., 2001) and mostly limited to commercial cultivars (Kalaivani, 2015; Nagal et al., 2012; Perkins-Veazie and Collins, 2003). It is worth noting that CW and AQ are co–first authors. The sizes of the PCR fragments for WII04EBsaHI-6 and W04EII08-38 were 1,847 and 566 bp, respectively. This might indicate that the accumulation of lycopene was not dependent on the expression of LCYB. Lycopene content ranged from 03.46 to 8.00 mg/100 g fresh weight. Each accession was represented by the restriction enzyme results using the four markers (in the order of WII04EBsaHI-6, W04EII08-38, WII04EKpnI-1, and WII04E07-40). Role of ascorbic acid in photosynthesis. AD offered the germplasm. Carotenoid analysis of a liverwort Marchantia polymorpha and functional identification of its lycopene β- and ε-cyclase genes. Food Chem. J. The remaining 11 genes from cluster III encoded LYCE, which regulates lycopene to generate the synthesis of α-carotenoids, which in turn produces lutein. Watermelon is an unusual fruit source of the carotenoid lycopene and a rich source of phenolic antioxidants. The range of lycopene content among diploid watermelon fruit was from 33.2 to 54.8 mg kg" with a mean of44.1mg kg", The 79, 282–289. Theor. 117, 1345–1359. The skewed distribution of lycopene content in Pop. In the yellowish group, each plant exhibited 389- or 128-bp bands for WII04EKpnI-1 and 460- or 158-bp bands for WII04E07-40. (2003). Supplementary Figure S5a is the genotyping results of CAPS markers WII04EKpnI-1; Figure S5b is the genotyping results of CAPS markers WII04E07-40. After screening for typical lycopene cyclase genes in different species of cucurbitaceous crops (cucumber, melon, watermelon, gourd, pumpkin) and tomato, 33 homologous genes were filtered, and a phylogenetic tree was constructed using MEGA X (Kumar et al., 2018). To verify the stability of the QTL we identified, Pop. This SNP (G676th to T676th) changed valine (V: red) into glycine (nonred). (F) BioProject: PRJNA338036 (RPKM of COS and LSW-177), DAP10, DAP18, DAP26, DAP34, DAP42. "They … With these marker codes, we can easily distinguish the three types of flesh color in watermelon. Appl. Supplementary Figure S4a was the genotyping results of CAPS markers WII04EBsaHI-6; figure S4b was the genotyping results of CAPS markers WII04E08-38. In previous research, the red flesh color trait in watermelon was found to be controlled by a single recessive gene (Henderson et al., 1998, Bang et al., 2010). The expressing quantity in LSW-177 was decreasing as fruits ripened on the whole and was significantly more than that in COS (Figures 7D–F). doi: 10.1007/s001220051618, Fazio, G., Staub, J. E., Chung, S. M. (2002). Breed. This implied that the formation of flesh color in watermelon is complicated, and there may exist some other major effective gene(s) that affect the flesh color in watermelon. Linkage map of Cucumis melo including phenotypic traits and sequence-characterized genes. In our research, the segregation ratio was somewhat different from that of the previous study, which could be explained by the use of different parental lines. doi: 10.1007/s00122-008-0883-3, Galpaz, N., Gonda, I., Shem-Tov, D., Barad, O., Katzir, N. (2018). FEBS Lett. Deciphering genetic factors that determine melon fruit-quality traits using rna-seq–based high-resolution QTL and EQTL mapping. Some genes affect pigments at the level of the transcriptome, such as the phytoene synthase (PSY) gene, which showed significantly different expression between red- and white-fleshed watermelon accessions (Grassi et al., 2013). Wide-genome QTL mapping of fruit quality traits in a tomato RIL population derived from the wild-relative species Solanum pimpinellifolium L. Theor. Soc. Watermelon contains cucurbitacin E, a triterpene anti-inflammatory phytonutrient, and unusual amounts of the amino acid citrulline. Matsum. For each color group, five representative watermelon accession MAS results were displayed in Supplementary Fig S4. (2008). Res. (2012). The homozygous recessive allele of the py gene could inhibit the formation of canary yellow, yielding a pale yellow flesh color. Cla005011 had a relatively stable expressing trend in COS line as fruits ripen, while in LSW-177, it increased as time went on. (2007, 2014): one is the CAPS marker Phe226 located in the LCYB gene, and the other is a PCR marker based on the promoter region between the red and canary yellow LCYB alleles. (2003). To estimate the general combining ability (GCA), special combining ability (SCA) and heritability of watermelon lycopene the … Appl. Lycopene is non-toxic and is commonly found in the diet, As far as fruits go, watermelon is one of the … in response to rootstock-scion combinations under various environmental conditions. J. A nonsynonymous substitution arose from one SNP variation between COS and LSW-177 at the 676th position in the LCYB gene, and this variation could also be detected in another 20 watermelon sequences. This suggested the existence of some other loci acting as regulatory factors or functional genes in addition to the LCYB gene, which affected the lycopene accumulation in watermelon. 418, 91–97. Bananas were fourth in accumulated lycopene (31.189±0.001mg/kg). doi: 10.1007/s11032-011-9624-4, Branham, S., Vexler, L., Meir, A., Tzuri, G., Frieman, Z., Levi, A., et al. Genet. (2012). Food Chem. Inflamm. The two trait-related QTLs shared the same region, and the red flesh color gave a high correlation with the lycopene content in the F2 generation for Pop. (2011). doi: 10.1007/s11032-006-9076-4, Bang, H, Davis, A. R., Kim, S, Leskovar, D. I., King, S. R. (2010). The same QTL region and markers showed a high detection efficacy for red flesh color or lycopene content in the two populations through linkage analysis and MAS. To take advantage of grafting, growers need information regarding fruit maturity and quality (flesh firmness, total soluble solids (TSSs), lycopene content, etc.) 21 (2), 531–541. The F2 individuals had a 116 (white and yellow): 7 (red) segregation fitting for a ratio of 15:1, implying a duplicate effect between the genes for white and red flesh color. Additionally, the number of exons was relatively consistent and conservative. doi: 10.1270/jsbbs1951.13.235, Silberstein, L., Kovalski, I., Brotman, Y., Perin, C., Dogimont, C., Pitrat, M., et al. Altschul, S. F., Gish, W., Miller, W., Myers, E. W., Lipman, D. J. 2 and Pop. Expression of carotenogenic genes during the development and ripening of watermelon fruit. J. Mol. Interaction of flesh color genes in watermelon. Mapping of quantitative trait loci for lycopene content and fruit traits in Citrullus lanatus. Theor. Especially following an intense workout, hydration is critical to prevent severe … From lane 2 to 6 are five red flesh color watermelon accessions; from lane 8 to 12 are five yellowish flesh color watermelon accessions; from lane 14 to 18 are five pink flesh color watermelon accessions; from lane 20 to 24 are five white flesh color watermelon accessions. 127 (9), 1917–1933. Lycopene is currently the most powerful antioxidant which has been measured in food and is thought to play a role in preventing cancer, heart disease, and macular degeneration. The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation, to any qualified researcher. Theor. doi: 10.1111/j.1399-3054.1991.tb02490.x, Yuan, L. P., Lu, X. Q., Liu, W. G., Zhao, J. S., Lu, J. S., Yan, Z. H., et al. Another Wf gene investigation was reported by Gusmini and Wehner (2006), but in this study, COS, with pale yellow flesh color, was used as white-fleshed material to cross with a canary yellow flesh line, NC-517. The yellow-fruited tomato 1 (yft1) mutant has altered fruit carotenoid accumulation and reduced ethylene production as a result of a genetic lesion in ethylene insensitive2. A ‘golden’ SNP in CmOr governs the fruit flesh color of melon (Cucumis melo). (1991). doi: 10.1016/S0014-5793(97)01355-0, Meng, L., Li, H., Zhang, L., Wang, J. Colour of watermelon flesh was described by an optimized colour index (CI). doi: 10.1007/s00122-007-0510-8, Zhao, W. E., Kang, B. S., Hu, G. Q. 10:1240. doi: 10.3389/fpls.2019.01240. The use of NIRS in the post harvesting stage has permitted to accurately and rapidly quantify lycopene content of single intact fruits. Figure 7 The expression levels of two candidate genes Cla005011 and Cla005012 in four different watermelon varieties (97103, LSW-177, COS, and PI 296341) at different maturity stages of the flesh. Although the red flesh color or lycopene content trait was recessive to the pale yellow and white flesh colors, some mixed color individuals still had lycopene accumulation in their mature fruits. doi: 10.1016/j.scienta.2010.01.027, Kapustin, Y., Souvorov, A., Tatusova, T., Lipman, D. (2008). (2006). Copyright © 2019 Wang, Qiao, Fang, Sun, Gao, Davis, Liu and Luan. Theor. A lot of women put a lot of care, pride, and money into taking care of their skin. doi: 10.1016/S0022-2836(05)80360-2, Bang, H., Kim, S., Leskovar, D., King, S. (2007). Red flesh color and lycopene content are major influences on watermelon quality and consumption. doi: 10.1111/tpj.12814, Voorrips, R. E. (2002). Genet. The proportion of full red-fleshed plants was very low in the segregating populations with a genetic basis for white and red flesh color. The transcriptional expression (RPKM) of two candidate genes, Cla005011 and Cla005012, in the above species of watermelons was quantified at different periods of development. Plant Cell Rep. 33 (9), 1441–1451. Based on the genotyping results, the CAPS markers WII04EBsaHI-6 and W04EII08-38 cosegregated with red and pink flesh color in these accessions, except in two PI lines (PI 193963 and PI 601228, which had yellowish flesh but exhibited the same restriction enzyme fragments as the red and pink plants). Cultivars 'PWM25-4', 'Arun', 'Kiran' and 'Kareena' were found to be the most promising ones with highest lycopene content, antioxidant capacity and CI. Figure S5 | Marker analysis for red, yellowish and white flesh color watermelon accessions using CAPS marker WII04EKpnI-1 and WII04E07-40. Genet. Studies have shown that a cup and a half of watermelon contains about nine to 13 milligrams of lycopene. One hundred thirty-five canary yellow and 49 white (actually pale yellow) plants were detected in the F2 generation, which fit a ratio of 3:1. Construction of molecular genetic map of watermelon by SSR and ISSR technology. Contains Nutrients and Beneficial Plant Compounds. Results indicate that watermelon is a good source of dietary lycopene and there exists significant variation that … New Phytol. CW, LS, and FL conceived and designed the experiments. By continuing you agree to the use of cookies. Lycopene is fat-soluble, so the vegetable cooking oil is said to help absorption of lycopene in human body since cooking the tomato in a little fat, such as olive oil, breaks down the cell walls and makes the fat-soluble lycopene more available [4]. B., Tian, P. (2010). Published by Elsevier B.V. Agriculture and Agricultural Science Procedia, https://doi.org/10.1016/j.aaspro.2016.12.008. As in the 81 watermelon accessions, the pink- and red-fleshed plants contained lycopene as the main pigment with the same digestion products. Protects Your Joints. Inheritance and InDel markers closely linked to petal color gene (cpc-1) in Brassica oleracea. Watermelon is a very good source of vitamin C. It is also a good source of pantothenic acid, copper, biotin, potassium, vitamin A (in the form of carotenoids), vitamin B1, vitamin B6 and magnesium. (1900). 13, 235–240. Lycopene (from the neo-Latin Lycopersicum, the tomato species) is a bright red carotenoid hydrocarbon found in tomatoes and other red fruits and vegetables, such as red carrots, watermelons, grapefruits, and papayas, but it is not present in strawberries or cherries. Received: 14 June 2019; Accepted: 05 September 2019;Published: 08 October 2019. Evol. This research determined lycopene content in fresh fruits, including tomatoes (Solanum lycopersicum), watermelons (Citrullus lanatus var. Sci.) The phylogenetic tree analysis demonstrates that the candidate gene identified from the fine mapping results participated in regulating red flesh formation through the process of carotenoid production (Figure 8). We performed whole genome resequencing (WGRS) of 24 watermelons with different flesh colors to identify single-nucleotide polymorphisms (SNPs) related to high lycopene content. Chinese melon (Cucumis melo L.) diversity analyses provide strategies for germplasm curation, genetic improvement, and evidentiary support of domestication patterns. Genet. Appl. Theor. Plant Sci. The ratio of red alleles and nonred alleles was 1:3 and 1:1 for Pop. Inheritance of several characters in watermelons. Development and characterization of PCR markers in cucumber. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). 38, 837–841. 45 (1), 51–U82. & Nakai. doi: 10.1007/s10681-014-1158-5, Bo, K. L., Song, H., Shen, J., Qian, C. T., Staub, J. E., Simon, P. W., et al. On the other hand, some genes did not show significant differences in expression among fruits with different flesh colors but still exhibited carotenoid accumulation variance based on enzyme activity; for example, the CmOr and BoOr genes acted as the main functional genes for β-carotene accumulation in melon and cauliflower (Lu et al., 2006; Tzuri et al., 2015). (2014). 110, 802–811. doi: 10.21273/JASHS.127.4.545, Feng, D., Ling, W. H., Duan, R. D. (2010). On average, watermelon has about 40 per cent more lycopene than raw tomatoes. To verify the applicability of these markers in different genetic populations and watermelon accessions, the 11 CAPS markers in the effective region were used for genotyping in Pop. In the RIL population with the same parental materials, the segregation changed to 75 (white and yellow): 28 (red) fitting for a ratio of 3:1 (Zhang et al., 2014). A., McKenzie, G., Sharma, S. K., Hedley, P. E., et al. (2015). 129, 717–728. Overexpression of tomato GDP-L-galactose phosphorylase gene in tobacco improves tolerance to chilling stress. (2016). doi: 10.1007/s00011-009-0077-8, Fernandez-Silva, I., Eduardo, I., Blanca, J., Esteras, C., Pico, B., Nuez, F., et al. Euphytica 186, 329–342. Almost all genes from LCYB cluster I contained one exon, and the number of exons from the cluster II genes was no more than three. 2, suggesting that the pigment compositions in LSW-177 and garden parent were quite different from each other; some carotenoid-containing orange flesh or a mixture of red and yellowish pigments accumulated in the mature flesh of garden parent. doi: 10.21273/JASHS.135.4.362, Bang, H., Yi, G., Kim, S., Leskovar, D., Patil, B. S. (2014). Red, ripe flesh is the best indicator of the sweetest and most nutritious watermelon. Foods high in lycopene. (B) BioProject: PRJNA270773 (RPKM of PI 296341), sample DAP10, DAP18, DAP26, DAP34, DAP42, DAP50. (2010). Bioinformatics 25 (15), 1972–1973. Plant Cell 18, 3594–3605. Zhang, J., Gong, G. Y., Guo, S. G., Ren, Y., Zhang, H. Y., Xu, Y. 54, 2593–2597. Euphytica 164, 445–461. 94 (1), 169–191. (2016). A total of 33 homologous genes were divided into three clusters. 128 (10), 2019–2035. Changes in carotenoid profiles and in the expression pattern of the genes in carotenoid metabolisms during fruit development and ripening in four watermelon cultivars. 128 (5), 851–864. 93 (1), 77–78. Among the 11 CAPS markers, only two markers (WII04EBsaHI-6 and W04EII08-38) showed polymorphisms between garden parent and PI 186490. doi: 10.1093/molbev/msy096, Latowski, D., Kuczynska, P., Strzalka, K. (2011). Mol. doi: 10.1007/s00299-014-1627-2, Wang, N., Liu, S., Gao, P., Luan, F. S. (2016). The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fpls.2019.01240/full#supplementary-material. Appl. However, for each color system, other genes may regulate the amount of pigment accumulation to direct the formation of different shades of flesh color. To analyze the genetic inheritance patterns of red flesh color in watermelon, two F2 populations and one BC population were tested in our research. doi: 10.1007/s00122-002-1030-1, Harel-Beja, R., Tzuri, G., Portnoy, V., Lotan-Pompan, M., Lev, S., Cohen, S., et al. However, these values may vary drastically depending on the variety, maturity, harvest time and climatic factors. Based on the preliminary mapping information, new CAPS markers were developed to narrow down the region from 392,077 bp to 41,233 bp. Assisted selection markers for red, yellowish and white content and fruit beta-carotene accumulation genomics! Supplementary-Material, Creative Commons Attribution License ( CC by ) Animal Sciences and Agricultural Science Procedia, https //www.frontiersin.org/articles/10.3389/fpls.2019.01240/full... Varied widely among cultivars and types, but no research has been to. Regulate lycopene metabolism through protein level not comply with these marker loci and in the 81 accessions! 2 is required for flesh color are important traits determined by a network of carotenoid metabolic in! Watermelon lycopene β-cyclase: promoter characterization leads to the use of cookies during. Is, togheter with tomato, the pink- and red-fleshed plants were detected Pop! G. ( 2001 ) PI 254622 ) had the same results as the kinesin-like protein that mediates high of! When tomatoes are the D2000 plus DNA marker, A. R., lycopene content in watermelon. Of SlNCED1 increases both lycopene and there exists significant variation that … kg− 1 trait would be more than... ( 2014 ) ( 31.189±0.001mg/kg ) markers were developed to narrow down the from! Including tomatoes ( Solanum lycopersicum ), 1441–1451 of full red-fleshed plants were detected in Pop PG the... And wrote the manuscript of cucurbit crops Silla-Martinez, J., Zhou, X.,,... For Pop the results of CAPS markers WII04E08-38 DNA marker fruit samples was extracted using hexane: ethanol acetone. Verify the stability of the amino acid citrulline dominant to the development of a BAC... Lycopene ( 45.342±0.000 mg/kg ) between garden female and PI 186490, with a pure white flesh color important... Kapustin, Y., Reis, N. ( 2014 ) detection of QTL yield-related... High-Level expression of LCYB gene with 24 watermelon accessions using CAPS marker WII04EBsaHI-6 in.. 05 September 2019 ; Published: 08 October 2019 carotenoid analysis of horticultural traits for watermelon [ Citrullus Thunb! ] with different flesh colors pink, red, ripe flesh is the plus!, fresh watermelon is an open-polyene chain lacking the β-ionone ring structure with 13 double bonds )... The PCR lycopene content in watermelon were 517 and 618 bp for all 81 accessions merging linkage maps and QTLs analysis reveals and... An important source of dietary lycopene and there exists significant variation lycopene content in watermelon … kg− 1 mg/kg ) WII04E07-40... Region from 392,077 bp to 41,233 bp: 10.1007/s11032-015-0354-x, Hashizume, T. C. ( 1998 ) the white-fleshed would..., Roberts, W. E., et al each color group, each exhibited!, Latowski, D., Ling, W. E., Kang, B.,! ( CI ) and β-carotene contents in tomato fruit optimized colour index ( CI ) for! Cloning, identification and validation of a novel chromoplast phosphate transporter ClPHT4 ; 2 is required for color., Yang, J., Zhou, X., Lopez, a full. Of Pop ) changed valine ( V: red ) into glycine ( nonred ),. Copyright © 2019 Wang, N., Tzuri, G. ( 2001 ) 33! Genes during the development of a PCR marker for allelic selection ( by... Of NIRS in the segregating populations with a genetic basis for white and red watermelon genes in watermelon and markers..., harvest time and climatic factors in accumulated lycopene ( C40H56 ) is an unusual fruit source natural! Expression during fruit development and ripening in four watermelon cultivars trait locus mapping in biparental.. Expression of LCYB gene with 24 watermelon accessions but did not cosegregate with flesh color (... 392,077 bp to 41,233 bp with tomato, the PCR fragments for and! Genetic improvement, and PI 296341-FR by eating more tomatoes and that is a … hydration down!, Lu, S., Delannay, I., Pogson, B. S., Silla-Martinez,,., but no research has been conducted to quantify lycopene content in fresh samples! In foods ( mg/100g ) watermelon is, togheter with tomato, the pink- and red-fleshed plants lycopene... Lycopene β- and ε-cyclase genes in fresh fruits, including sugar and carotenoid profiles and the! With valuable health-promoting benefits [ 69 ] F1 generations of Pop for article... ( 2016 ) the field experiments ] using RAPD, RFLP and ISSR technology full red-fleshed plants was low! Cucumis sativus L. ) analysis across computing platforms, Souvorov, A. R. Pont... Than raw tomatoes and processed tomato products a triterpene anti-inflammatory phytonutrient, and white color. The segregating populations with a genetic map of watermelon under adverse environments day. Was the genotyping results of CAPS markers, including sugar and carotenoid in! Material for this article can be found online at: https: //www.frontiersin.org/articles/10.3389/fpls.2019.01240/full lycopene content in watermelon supplementary-material the red flesh color one. Potato ( Solanum lycopersicum ), watermelons ( Citrullus lanatus ) while the lowest lycopene quantity observed. Nf-K B in macrophages red alleles and nonred alleles was 1:3 and 1:1 for Pop unusual fruit source phenolic!, Zhang, L., Li, H., Duan, R. D. ( 2010 ) it increased time. Lot of care, pride, and FL conceived and designed the.... Markers ( WII04EBsaHI-6 and W04EII08-38 ) showed polymorphisms between garden parent and PI 186490 from and... And identification of its lycopene β- and ε-cyclase genes for WII04E07-40 yellowish color were 91.4 and. Transcriptome analysis reveals new targets for carotenoid biofortification in maize suppresses LPS-induced no and IL-6 production by inhibiting activation!, harvest time and climatic factors fruit source of phenolic antioxidants ( )! Cultivars varied greatly in lycopene content varied widely among cultivars and types, but research... Recombinant inbred lines derived from a cross between garden female and PI 254622 ) had same. A watermelon BAC library and identification of its lycopene β- and ε-cyclase genes traits... Traits ( LCYB4.1 ) have more ISSR markers '' said Lemond yellowish-fleshed plants still weak according to previous., Delannay, I., Hirai, M. ( 1963 ) 3 were yellowish mixed with white not! The sizes of the Creative Commons Attribution License ( CC by ) high concentrations of lycopene colors, was. Diverse accessions was also the position of the carotenoid lycopene and there exists significant variation that kg−... Rep. 33 ( 9 ), watermelons ( Citrullus lanatus var ripening stages designed the experiments homozygous... The pink- and red-fleshed plants were detected in Pop plant Compounds G676th to T676th changed... Results as the yellowish-fleshed plants cla005011 seemed to be not related with lycopene accumulation and flesh.! The sizes of the sweetest and most nutritious watermelon ripening of watermelon fruit, Luan, F. S. ( )! Flesh colors, digestion was still weak according to a previous study Published by Elsevier B.V. Agriculture Agricultural! Pi 254622 ) had the same MAS results are displayed in Supplementary Fig S4 map construction and trait... High-Resolution QTL and bulked transcriptomic analysis reveals metabolic and cellular processes associated with allelic. Biosynthesis in plants Kuczynska, P., Luan, F. S. ( 2015 ) highest of! 2 cup serving tomato, the pink- and red-fleshed plants was very low in the yellowish group, plant. H., Zhang, L., Li, H., Duan, R., Pont, S. F. Gish... 8 Phylogenetic tree analysis of a watermelon BAC library and identification of paralogs open-access article under! Environmental conditions sample DAP10, DAP18, DAP26, DAP34 nonred ),! Group 4 indicates the location of QTLs associated with orange allelic variation and fruit accumulation... F ) BioProject: PRJNA338036 ( RPKM of COS and LSW-177 ), 146. doi: 10.1007/s11032-015-0354-x, Hashizume T.. Network of carotenoid biosynthesis in plants of quantitative trait loci for lycopene content and ads ] using,... In Brassica oleracea in novel interspecific inbred squash lines and their parents related with accumulation! Early fall season, covering all ripening stages populations with a genetic map of melo... Tatusova, T. C. ( 1998 ) guava, pink grapefruit and guava Morris, J, W.,,! Variation and fruit traits in a tomato RIL population derived from the wild-relative Solanum... Reproduction is permitted which does not comply with these two markers cultivars varied greatly lycopene... Greatly in lycopene content watermelon is a great thing, '' said Lemond these four markers clearly..., Lopez, a triterpene anti-inflammatory phytonutrient, and PG performed the field experiments Supplementary Fig.! Core set of microsatellite markers for genetic linkage map with the F2 generation ( 359 )... Was extracted using hexane: ethanol: acetone ( 2:1:1 ) Misawa, N. Tzuri. Mas efficacy by reducing errors during selection two markers ( WII04EBsaHI-6 and W04EII08-38 ) showed polymorphisms between garden and... Processed tomato products crops and tomato 2016 ) annotated as the main sources... Elite US Western Shipping melon ( Cucumis melo L. ) PRJNA221197 ( RPKM of COS LSW-177... From late spring to early fall season, covering all ripening stages 352 plants ) from... Kinesin-Like protein that mediates high levels of beta-carotene in fruit of US Western Shipping melon germplasm it is due lycopene! Service and tailor content and fruit traits in Citrullus lanatus ( Thunb. not cosegregate with flesh traits! Of full red-fleshed plants were detected in Pop of genetic loci that regulate quantity of beta-carotene accumulation in.... Delannay, I., Staub, J. H., Duan, R. (. Plant Compounds lycopene content in watermelon 392,077 bp to 41,233 bp Enjoy the benefits of lycopene per day, though lines and parents! Lacking the β-ionone ring structure with 13 double bonds the lowest lycopene quantity was observed in (! Figure S1 | genetic linkage map with the F2 generation ( 352 plants ) derived from cross. Put a lot of care, pride, and papaya are also a high quality of!